Food, drug, insect sting allergy, and anaphylaxisCorrelation of serum allergy (IgE) tests performed by different assay systems
Section snippets
Patients
Infants/children and adolescents from 6 months to 21 years of age who were evaluated at the Mount Sinai Pediatric Allergy and Immunology practice and who had blood drawn for routine management were eligible to participate in this study. The patient history and physical examination, skin prick tests, and blood tests for allergen specific IgE levels were performed as part of standard clinical care. Written informed consent was obtained before enrollment. An additional blood sample (15 mL) was
Patients
Fifty consecutive patients who consented to participation were enrolled. The median age of the participants was 7.25 years (range, 2.92-18 years). Thirty-three were male and 17 were female. This was a highly atopic population with 42 diagnosed with food hypersensitivities (5 other patients avoided specific foods because of a history of positive skin prick tests or serum-specific IgE levels, and 3 patients had no history of food hypersensitivity), 42 with allergic rhinitis, 30 with atopic
Discussion
Measurement of allergen-specific IgE levels is important in the diagnosis of clinical allergy as well as the management of individual patients. We compared IgE levels measured by ImmunoCAP, our reference standard, with those measured by Immulite and Turbo-MP. Consistent with previous studies,6, 7, 8, 9 our data demonstrate significant differences in the measurement of IgE levels in identical serum samples using these 3 commercial assay systems. In our study, allergen-specific IgE levels
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2022, Allergic and Immunologic Diseases: A Practical Guide to the Evaluation, Diagnosis and Management of Allergic and Immunologic Diseases
All costs for the laboratory analysis were covered by Phadia AD, Uppsala, Sweden.
Disclosure of potential conflict of interest: J. Wang has received research support from Phadia. H. A. Sampson has consulting arrangements with Allertein and has received research support from Phadia and the National Institutes of Health. J. H. Godbold has declared that he has no conflict of interest.